• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Procedure to reduce contaminants in plant protein. The present invention relates to a process for reducing pollutants of inorganic (heavy metals) and organic (aflatoxins, molds, etc.) in vegetable matter with high protein content, mainly from rice. When applying this procedure on the vegetable raw material, derivatives are obtained that present levels of these contaminants well below the legal limits established by the sanitary authorities. By means of the process of the present invention high quality protein derivatives can be used for vitamin and nutritional supplements, infant feeding formulas, sports nutrition, clinical nutrition, ingredients in animal feed, etc. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2559902A1
    申请号:ES201531627
    申请日:2015-11-11
    公开日:2016-02-16
    发明作者:Iñaki MIELGO IZA；José Carlos MÁRQUEZ LÓPEZ；Eduardo J. ROMERO RAMÍREZ
    申请人:Pevesa Biotech SA；
    IPC主号:
    专利说明:

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    Pollutant reduction procedure in protein plant matter
    DESCRIPTION
    The present invention relates to a process for reducing contaminants of inorganic (heavy metals) and organic (aflatoxins, molds, etc.) origin in plant matter with high protein content, mainly from rice. When applying said procedure on the vegetable raw material, derivatives are obtained that have levels of these pollutants that are much lower, being able to process contaminated material and resulting in a product below the limits established for said pollutants. Through the process of the present invention high quality protein derivatives usable for vitamin and nutritional supplements, infant feeding formulas, animal feed ingredients, etc. are obtained.
    STATE OF THE TECHNIQUE
    The use of plant derivatives (in particular isolated, concentrated and hydrolyzed proteins) in various fields is common for several uses. However, there are risks associated with the presence of pollutants in them, as well as strict regulations for certain cases such as the current European legislation, in particular 1881/2006 (including its latest modifications such as the recent 488/2014 that regulates the maximum limits of cadmium in various foods) or EFSA recommendations for arsenic. In this sense, the use of plant derivatives is compromised by the presence of contaminants of an organic or inorganic nature. In order to obtain final products according to these strict requirements, it is necessary to opt for starting products that present a guarantee on the level of contaminants that they incorporate.
    There are many examples related to the absorption of metals and proteins as described in US4355132, which describes a method of extracting heavy metals using a framework of plant-based proteins, as well as various methods of recovering the metals of interest, as described in DE10050873.
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    Methods for purification of plant raw material are rare and generally complicated as described in KR20030061871, and usually focus on removing a single concrete metal.
    On the other hand, the methods to detoxify mycotoxins are usually complicated as described in CN102940131, in which an agent containing a mixture of algae, yeasts and clays is used and subsequently removed. Alternatively, methods are applied that are simpler but require the use of undesirable substances such as ammonia, as described in NL9000367.
    Therefore, there is a clear need for simple, mutually compatible and versatile processes that eliminate problematic contaminants and whose maximum presence in consumer products should be controlled.
    DESCRIPTION OF THE INVENTION
    Therefore, in a first aspect, the present invention relates to a process for reducing contaminants in protein plant matter which comprises performing each of the following steps at least once:
    a) mix the starting plant material with water and with at least one type of protease enzyme, bring this mixture to a pH between 3 and 10 and keep stirring for at least 20 minutes at a temperature between 20 to 90 ° C,
    b) perform a solid-liquid separation of the product obtained in the previous stage,
    c) optionally, subjecting the hydrolyzate obtained in the previous stage to a sterilization process.
    The protease enzyme may be any known to one skilled in the art and capable of functioning at the conditions to be achieved in the medium. They can be of natural origin or obtained from different biotechnological processes, and the conditions must be adjusted according to the catalyst used.
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    In a preferred embodiment, the pH of the mixture of step (a) is 5 to 8. In a more preferred embodiment the pH is 7.5.
    In another preferred embodiment the agitation time of step (a) is 60 to 180 minutes. In a more preferred embodiment, the stirring time of step (a) is 70 minutes.
    In another preferred embodiment the temperature of step (a) is between 50 to 80 ° C. In an even more preferred embodiment the temperature in step (a) is 60 ° C.
    In a more preferred embodiment, the sterilization process of step (c) is selected from uperization or pasteurization, although other sterilization processes known in the art are also applicable.
    Another aspect of the invention relates to an alkaline treatment process comprising performing each of the following steps at least once:
    a1) mix the starting plant material with water in the presence of hydrogen peroxide, bring this mixture to a pH higher than 9 and keep it under stirring for a time of at least 20 minutes, at a temperature between 75 to 95 ° C and
    b1) optionally, wash and solid-liquid separation of the product obtained in a1).
    In a preferred embodiment the stirring time of the mixture of step (a1) is 60 minutes.
    In another preferred embodiment the temperature of step (a1) is 85 to 90 ° C.
    In a preferred embodiment, this alkaline treatment procedure is performed prior to the enzymatic hydrolysis procedure described above.
    Another aspect of the invention relates to a neutral washing process comprising performing each of the following steps at least once:
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    a2) mix the starting plant material with water, bring this mixture to a pH of between 6.5 and 8.5 and keep it under stirring for a time of between 20 and 60 minutes, at a temperature of between 60 and 90 ° C ,
    b2) perform a washing and solid-liquid separation.
    In a more preferred embodiment, the stirring time of step (a2) is 30 minutes.
    In another more preferred embodiment the temperature of step (a2) is 60 to 80 ° C. In an even more preferred embodiment the temperature of step (a2) is 66 ° C.
    In another more preferred embodiment the pH of step (a2) is between 7 and 8.
    In another preferred embodiment, the neutral washing procedure is performed prior to the enzymatic hydrolysis procedure or the alkaline washing procedure described above.
    Another aspect of the invention relates to an acid washing process which comprises performing each of the following steps at least once:
    a3) mix the starting plant material with water, bring this mixture to a pH of between 2 and 4 and keep it under stirring for a time of between 20 and 60 minutes, b3) perform a washing and a solid-liquid separation.
    In a more preferred embodiment the pH of the mixture of step (a3) is 3.5 to 3.7.
    In another more preferred embodiment, the stirring time of step (a3) is 30 minutes.
    In another more preferred embodiment the temperature of step (a3) is controlled between 60 and 95 ° C.
    In another more preferred embodiment it is 80 ° C.
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    In another more preferred embodiment, the acid washing procedure is performed prior to the enzymatic hydrolysis procedure or the alkaline washing procedure or the neutral washing procedure described above.
    In the present invention solid-liquid separation is understood as any process of this type known to any person skilled in the art. Examples of solid-liquid separation processes applicable to the methods of the invention are decantation, precipitation, sedimentation, sieving, centrifugation filtration and cyclone, evaporation or drying. Preferably the solid-liquid separation is carried out by centrifugation.
    In a preferred embodiment the vegetable matter comes from rice, wheat, soy, corn, pea, carob, sunflower, potato, cotton, lentil or chickpea. In a more preferred embodiment, the starting plant material is rice.
    In a preferred embodiment the pollutant to be reduced is selected from metals, mycotoxins or microorganisms.
    In a more preferred embodiment, the metals are selected from heavy metals and other minerals. Non-limiting examples of metals are As, Cd, Pb, Hg, Al, Mo or V.
    In another more preferred embodiment the mycotoxin is selected from fumonisin B1, deoxynivalenol, ocratoxin A, aflatoxins B1, B2, G1, G2, M1, M2.
    In a more preferred embodiment the microorganisms are selected from molds, yeasts or bacteria. Non-limiting examples of microorganisms are E. coli, Salmonella, Cronobacter Sakazaaki, Bacillus cereus, Staphilococcus aerus or Lysteria.
    The different procedures of acid washing, neutral washing and alkaline treatment can be carried out alone or in combination and in any order prior to the main procedure of the invention of enzymatic hydrolysis according to the contaminant or specific contaminants that want to be removed from the starting material.
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    The values given for the conditions of temperature, time and pH can vary in practice by ± 0.5 for pH, ± 3 for temperature and ± 10 for time, because, due to the different chemical reactions and / or biochemical produced during the processes, it is difficult to maintain these parameters in such concrete values.
    Throughout the description and claims the word "comprises" and its variants are not intended to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and characteristics of the invention will be derived partly from the description and partly from the practice of the invention. The following examples and figures are provided by way of illustration, and are not intended to be limiting of the present invention.
    BRIEF DESCRIPTION OF THE FIGURES
    FIG. 1: Schematic description of the entire process.
    FIG. 2: Detailed description of the entire process.
    EXAMPLES
    The invention will be illustrated below by tests carried out by the inventors, which shows the effectiveness of the product of the invention.
    Heavy metal reduction procedure
    To prepare the starting raw material of the process, 150 kg of rice protein (raw material) were taken and suspended in 2,500 L of water.
    This mixture was stabilized at 19 ° C for 30 minutes and the pH was corrected to a value between 3.5-3.7 (+/- 0.5) using HCl. Specifically, the initial pH of the mixture was 4.76 and a final pH of 3.68 was achieved using 2.5 HCl. This mixture was separated using a horizontal centrifuge (decanter).
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    The resulting heavy phase (the solid of the first wash), was used for a second wash. For this, 2,500 L of water were added, heated under stirring at 66 ° C and the pH corrected to 7.0-8.0 (+/- 0.5) using 80% potash, stirring was maintained until stabilization. of pH. Specifically, 4.4 kg of potash were added to change from an initial pH of 4.08 to a final pH of 7.31. After this process a new solid-liquid separation was performed using a horizontal centrifuge (decanter).
    The resulting heavy phase (the solid from the second wash) was used as a substrate for enzymatic hydrolysis. For this, 2,500 L of water were added, stabilized at 60 ° C and the pH was corrected from an initial pH of 6.75 to a final pH of 7.59 by adding 1 L of 50% NaOH. 1 L of protease was added and the conditions were maintained for 70 minutes. The initial pH was 7.59 and the final pH 6.79. The enzyme was deactivated by raising the temperature to 85 ° C and maintaining it for 30 minutes.
    Subsequently, a separation was made by filtration in basket centrifuge and another clarification of the liquid using a plate centrifuge. The resulting light phase was stabilized by evaporation and spray drying. This powder is the final product low in contaminants (final product).
    Determination of contaminants in the samples obtained during the process.
    A sample of the final product powder was taken and the heavy metals indicated in Table 1 (ICP-Vis) were quantitatively determined by standard methods. The results obtained indicate that there is an independent fractionation of each of the metals during the process. The results obtained (in mg / 100g) for the product in comparison with the maximum limit (LIM.) Are detailed below:
     Element  Raw material Solid of the first wash Solid of the second wash Final product LIM.
     Pb  0.007 0.010 0.004 0.005 0.005
     CD  0.023 0.004 0.004 0.002 0.005
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     Ace  0.006 0.002 0.002 0.005 0.01
     Hg  0.002 0.001 0.002 0.001 0.005
     To the  1.22 1.44 1.03 0.412 3
    Table 1
    As can be seen in the data, the acid pH washing process significantly reduces Cd and As, the neutral pH washing significantly reduces Pb and As and the hydrolysis and clarification process significantly reduces the value of Cd, Hg and Al.
    Reduction procedure of Aflatoxin B1 and aerobic mesophylls
    To prepare the starting raw material of the process, 300 kg of rice protein (raw material) were taken and suspended in 3,100 L of water.
    This mixture was stabilized at 85 ° C for 60 minutes and the pH was corrected to a value between 9 (+/- 0.2) using NaOH. Specifically, the initial pH of the mixture was 5.42 and a final pH of 9.15 was achieved using 5Kg of NaOH (50%), hydrogen peroxide was added up to 10L.
    The resulting mixture was used as a substrate for enzymatic hydrolysis. For this, it was stabilized at 60 ° C and the pH was corrected from an initial pH of 9.15 to a final pH of 8.1 by adding 1 L of 35% HCL. 1.2 L of protease was added and the conditions were maintained for 80 minutes. The initial pH was 8.1 and the final pH 5.93. The enzyme was deactivated by raising the temperature to 85 ° C and maintaining it for 30 minutes.
    Subsequently, a separation by filtration in basket centrifuge and another clarification of the liquid was performed using a plate centrifuge. The resulting light phase was concentrated by evaporation.
    The resulting syrup was subjected to pasteurization in a tank in which it was heated to 80-85 ° C under agitation until the H2O2 was removed with a minimum of 3 hours
    Finally the product is stabilized in spray drying. This powder is the final product low in aflatoxins and microbiology (final product).
    5 Determination of contaminants in the samples obtained during the process.
    A sample of the final product powder was taken and the aflatoxins and the aerobic content 10 mesophils indicated in Table 2 were quantitatively determined by standard methods. The results obtained indicate that there is a reduction in aflatoxin B1 and aerobic microorganisms mesophiles during the process. The results obtained are detailed below (in ppb for aflatoxins and in cfu / g for mesophilic aerobes):
     Substance  Raw material Final product LIM.
     Aflatoxin B1  2.3 0.05 0.3
     Aflatoxin B2  <0.050 <0.050 0.3
     Aflatoxin G1  <0.050 <0.050 0.3
     Aflatoxin G2  <0.050 <0.050 0.3
     Aerobic mesophilic  2,500 1,800 10,000
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    Table 2
    As can be seen in the data, the alkaline treatment significantly reduces aflatoxin B1 and the pasteurization process reduces the microbial load.
    权利要求:
    Claims (30)
    [1]
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    1. Procedure for the reduction of contaminants in protein plant matter, which involves performing each of the following stages at least once:
    a) mix the starting plant material with water and with at least one type of protease enzyme, bring this mixture to a pH of between 3 and 10 and keep under stirring for a time of at least 20 minutes, at a temperature of between 20 at 90 ° C,
    b) optionally, perform a solid-liquid separation of the product obtained in the previous stage,
    c) optionally, subjecting the hydrolyzate obtained in the previous stage to a sterilization process.
    [2]
    2. Method according to claim 1 wherein the pH of the mixture of step (a) is between 5 and 8.
    [3]
    3. Method according to the preceding claim wherein the pH of the mixture of step (a) is 7.5.
    [4]
    4. Method according to any of the preceding claims wherein the stirring time of step (a) is 60 to 180 minutes.
    [5]
    5. Procedure according to the previous claim where the stirring time of step (a) is 70 minutes.
    [6]
    6. Method according to any of the preceding claims wherein the temperature of step (a) is between 50 to 80 ° C.
    [7]
    7. Method according to the previous claim where the temperature in step (a) is 60 ° C.
    [8]
    8. Method according to any of the preceding claims wherein the sterilization process of step (c) is selected from uperization or pasteurization.
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    [9]
    9. Procedure for reducing pollutants in protein plant matter, which involves performing each of the following stages at least once:
    a1) mix the starting plant material with water in the presence of hydrogen peroxide, bring this mixture to a pH greater than 9 and keep it under agitation for a time of at least 20 minutes, at a temperature between 75 to 95 ° C and
    b1) optionally, perform a solid wash and liquid separation of the product obtained in a1).
    [10]
    10. Method according to claim 9 wherein the stirring time of the mixture of step (a1) is 60 minutes.
    [11]
    11. Method according to any of claims 9 or 10 wherein the temperature of step (a1) is 85 to 90 ° C.
    [12]
    12. Method according to any one of claims 9 to 11 which comprises performing step (a1) and optionally (b1) prior to step (a) of the method described according to any of claims 1 to 8.
    [13]
    13. Procedure for the reduction of contaminants in protein plant matter, which involves performing each of the following stages at least once:
    a2) mix the starting plant material with water, bring this mixture to a pH of between 6.5 and 8.5 and keep it under stirring for a time of between 20 and 60 minutes, at a temperature of between 60 and 90 ° C , b2) perform a washing and solid-liquid separation.
    [14]
    14. Method according to claim 13 wherein the pH of step (a2) is between 7 and 8.
    [15]
    15. Method according to any of claims 13 or 14 wherein the stirring time of step (a2) is 30 minutes.
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    [16]
    16. Method according to any of claims 13 to 15 wherein the temperature of step (a2) is 60 to 80 ° C.
    [17]
    17. Method according to the preceding claim wherein the temperature of step (a2) is 66 ° C.
    [18]
    18. A method according to any one of claims 13 to 17, comprising performing step (a2) and (b2) prior to step (a) of the method described according to any of claims 1 to 8 or prior to step (a1) of Process described according to any of claims 9 to 12.
    [19]
    19. Procedure for the reduction of contaminants in protein plant matter, which involves performing each of the following stages at least once:
    a3) mix the starting plant material with water, bring this mixture to a pH of between 2 and 4 and keep it under stirring for a time of between 20 and 60 minutes, and
    b3) perform a washing and solid-liquid separation.
    [20]
    20. Method according to the preceding claim wherein the pH of the mixture of step (a3) is 3.5 to 3.7.
    [21]
    21. Method according to any of claims 19 or 20 wherein the stirring time of step (a3) is 30 minutes.
    [22]
    22. Method according to any of claims 19 to 21 wherein the temperature of step (a3) is controlled between 60 and 95 ° C.
    [23]
    23. Method according to the previous claim where the temperature of step (a3) is 80 ° C.
    [24]
    24. Method according to any one of claims 19 to 23 which comprises performing step (a3) and (b3) prior to step (a) of the method described according to any of claims 1 to 8 or
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    prior to step (a1) of the process described according to any of claims 9 to 12 or prior to step (a2) of the process described according to any of claims 13 to 18.
    [25]
    25. Method according to any of the preceding claims wherein the vegetable matter comes from rice, wheat, soy, corn, pea, carob, sunflower, potato, cotton, lentil or chickpea.
    [26]
    26. Method according to any of the preceding claims wherein the contaminant to be reduced is selected from metals, mycotoxins or microorganisms.
    [27]
    27. Method according to the previous claim where the metals are selected from As, Cd, Pb, Hg, Al, Mo or V.
    [28]
    28. Method according to claim 26 wherein the mycotoxin is selected from fumonisin B1, deoxynivalenol, ocratoxin A, aflatoxins B1, B2, G1, G2, M1, M2.
    [29]
    29. Method according to claim 26 wherein the microorganisms are selected from molds, yeasts or bacteria.
    [30]
    30. Method according to the previous claim where the microorganisms are selected from among E. coli, Salmonella, Cronobacter Sakazaaki, Bacillus cereus, Staphilococcus aerus or Lysteria.
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    同族专利:
    公开号 | 公开日
    ES2559902B1|2016-11-22|
    WO2017081347A2|2017-05-18|
    US20190029295A1|2019-01-31|
    WO2017081347A3|2017-09-14|
    EP3400806A1|2018-11-14|
    EP3375290A2|2018-09-19|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    GB1117573A|1966-06-07|1968-06-19|Council Scient Ind Res|Improvements in or relating to the detoxification of foodstuffs containing aflatoxin|
    WO2011008276A2|2009-07-14|2011-01-20|The United States Of America, As Represented By The Secretary Of Agriculture|Utilization of non-nutritive absorbents to sequester mycotoxins during extraction of protein or other value added components from mycotoxin contaminated cereal or seed oil meal|
    CN103549234A|2013-10-23|2014-02-05|华中农业大学|Method of reducing heavy metals of cereals|WO2018178271A1|2017-03-31|2018-10-04|Compagnie Laitiere Europeenne|Hydrolysed vegetable proteins suitable for use in baby food|
    CN110618097A|2019-09-04|2019-12-27|广东省测试分析研究所（中国广州分析测试中心）|Mercury morphological analysis pyrolysis device with adjustable and controllable temperature and flow rate and use method|CA2020461A1|1989-07-14|1991-01-15|Donald J. Hamm|Process for the production of hydrolyzed vegetable proteins and the product therefrom|
    US5266338A|1991-04-15|1993-11-30|Nabisco, Inc.|Egg pasteurization|
    DE4133538C2|1991-10-10|1993-11-11|Waldemar Dr Neumueller|Process for the production of food-grade proteins from a protein-containing substance|
    TW589383B|1998-04-30|2004-06-01|Ajinomoto Kk|Method for producing hydrolyzed protein|
    ES2356607T3|2002-08-14|2011-04-11|Novozymes A/S|FEED COMPOSITION AND ANIMAL FEEDING METHOD.|
    CN101511211A|2006-02-02|2009-08-19|帝斯曼知识产权资产管理有限公司|Food product comprising a proline specific protease|
    法律状态:
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    ES201531627A|ES2559902B1|2015-11-11|2015-11-11|Procedure for reducing contaminants in protein plant matter|ES201531627A| ES2559902B1|2015-11-11|2015-11-11|Procedure for reducing contaminants in protein plant matter|
    PCT/ES2016/070797| WO2017081347A2|2015-11-11|2016-11-10|Procedure for reducing contaminants in vegetable protein matter|
    EP18181929.3A| EP3400806A1|2015-11-11|2016-11-10|Procedure for reducing contaminants in vegetable protein matter|
    US15/775,177| US20190029295A1|2015-11-11|2016-11-10|Procedure for reducing contaminants in vegetable protein matter|
    EP16813074.8A| EP3375290A2|2015-11-11|2016-11-10|Procedure for reducing contaminants in vegetable protein matter|
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